The aim of this study was to investigate and report potential improvements in the screening protocol that is currently applied in Romania in order to reduce the time between birth and treatment initiation in newborns positive for congenital hypothyroidism screening. Blood samples were collected (Cord blood and DBS) from 41 full-term newborns between February and March 2019 and analyzed using chemiluminescent microparticle immunoassay and fluorometric enzyme-linked immunoassay (Lab- systems Diagnostic, Oy, Finland), respectively. Based on the findings, an improvement to the existing protocol is proposed that may reduce the time from positive screening results to confirmation of congenital hypothyroidism and treatment initiation.