The recent surge in the diagnoses of congenital hypothyroidism (CH) has necessitated the measurement of newborn TSH levels and an understanding of the way various antenatal and perinatal factors influence its dynamicity. It is a cross-sectional study on newborns delivered or admitted to the All India Institute of Medical Sciences Raipur (AIIMS Raipur), Chhattisgarh, India. Dried blood spot analysis of newborn thyroid-stimulating hormone (nTSH) was carried out using fluoroimmunoassay kits (Labsystems Diagnostics Oy, VANTAA, Finland). The mean values and percentile observed in this study were comparable to the other study which used the time-resolved fluorescence immunoassay method. Higher nTSH was observed among newborns born to mothers with a thyroid disorder, those delivered prematurely and/or by cesarean section, and those with no antenatal visits.